SMAD3 antibody (Internal Region)
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- Target See all SMAD3 Antibodies
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SMAD3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-e70
- Supplier
- Rockland
- Purpose
- SMAD3 Antibody
- Cross-Reactivity (Details)
- SMAD3 Antibody is directed against human Smad3 protein.
- Characteristics
- Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2
- Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product SMAD3 Primary Antibody
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- Application Notes
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Application Note: SMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: 1:15,000-1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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Transforming growth factor-{beta}-inducible phosphorylation of Smad3." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9663-73, (2009) (PubMed).
: "
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Transforming growth factor-{beta}-inducible phosphorylation of Smad3." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9663-73, (2009) (PubMed).
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- Target
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- Alternative Name
- SMAD3 (SMAD3 Products)
- Background
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.
- Gene ID
- 4088
- NCBI Accession
- NP_005893
- UniProt
- P84022
- Pathways
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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