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SMAD3 antibody (Internal Region)

Rockland 600-401-e70 SMAD3 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN1043911
Supplier Product No.: 600-401-e70
  • Target See all SMAD3 Antibodies
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Binding Specificity
    • 38
    • 33
    • 26
    • 25
    • 24
    • 12
    • 11
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 227
    • 153
    • 135
    • 27
    • 9
    • 9
    • 8
    • 7
    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 219
    • 22
    Rabbit
    Clonality
    • 204
    • 37
    Polyclonal
    Conjugate
    • 119
    • 13
    • 12
    • 11
    • 8
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    This SMAD3 antibody is un-conjugated
    Application
    • 191
    • 95
    • 73
    • 72
    • 55
    • 53
    • 49
    • 49
    • 27
    • 13
    • 10
    • 8
    • 5
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-401-e70
    Supplier
    Rockland
    Purpose
    SMAD3 Antibody
    Cross-Reactivity (Details)
    SMAD3 Antibody is directed against human Smad3 protein.
    Characteristics
    Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2
    Purification
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: SMAD3 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein surrounding amino acid residue 179.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product SMAD3 Primary Antibody
  • Application Notes

    Application Note: SMAD3 Antibody has been tested for use in ELISA and by western blot. This antibody is suitable in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48.1 kDa in size corresponding to human Smad3 protein by western blotting in the appropriate tissue or cell lysate or extract.

    Western Blot Dilution: 1:1,000

    ELISA Dilution: 1:15,000-1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Wang, Matsuura, He, Liu: "Transforming growth factor-{beta}-inducible phosphorylation of Smad3." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9663-73, (2009) (PubMed).

  • Target
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Alternative Name
    SMAD3 (SMAD3 Products)
    Background
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Activated type I receptor phosphorylates receptor-activated Smads (R-Smads) at their c-terminal two extreme serines in the S-S-X-S motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the bone morphogenic protein or BMP pathway. Upon phosphorylation R-Smads are translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated response.
    Gene ID
    4088
    NCBI Accession
    NP_005893
    UniProt
    P84022
    Pathways
    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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