Glycogen Synthase 1 antibody (pSer641)

Catalog No. ABIN1043894

Supplier Product No.: 600-401-b28

Product Details anti-Glycogen Synthase 1 Antibody

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Binding Specificity: pSer641

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycogen Synthase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-401-b28

Supplier: Rockland

Purpose: Muscle Glycogen Synthase Phospho S641 Antibody

Cross-Reactivity (Details): Phospho Glycogen Synthase pS641 antibody is directed against human muscle glycogen synthase.

Characteristics: Synonyms: rabbit anti-muscle glycogen synthase pS641 antibody, rabbit anti-glycogen synthase pS641 antibody, Glycogen antibody, Glycogen synthase 1 (muscle) antibody, Glycogen synthase 1 antibody, Glycogen synthase1 antibody, GYS 1 antibody, GYS-1 antibody, GYS1 antibody, Starch synthase muscle antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to pS641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This phospho specific polyclonal antibody was tested by immunohistochemistry, immunoblotting, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store Phospho GYS1 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Glycogen Synthase 1 antibody (ABIN1043894)

Kelsall, Voss, Munro, Cuthbertson, Cohen: "R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals." in: Journal of neurochemistry, Vol. 118, Issue 4, pp. 596-610, (2011) (PubMed).

Target Details for Glycogen Synthase 1

Target: Glycogen Synthase 1 (GYS1) (Glycogen Synthase 1 (Muscle) (GYS1))

Alternative Name: GYS1 (GYS1 Products)

Background: Background: Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Gene ID: 2997

UniProt: P13807

Pathways: PI3K-Akt Signaling, AMPK Signaling, Cellular Glucan Metabolic Process