IL-27 antibody

Catalog No. ABIN1043835

Supplier Product No.: 210-501-b54

Product Details anti-IL-27 Antibody

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Multiplex Assay (MA)

Supplier Product No.: 210-501-b54

Supplier: Rockland

Purpose: Mouse IL-27/p28 Antibody

Cross-Reactivity (Details): This antibody is specific for mouse and rat p28 protein.

Characteristics: Synonyms: rat anti-IL-27/p28 antibody, rat anti-interleukin 27a antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

Purification: IL-27 / p28 antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 3H12-F10

Isotype: IgG2a kappa

Application Details

Application Notes:

Flow Cytometry Dilution: 1 μg / ml

Application Note: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL-27 antibody (ABIN1043835)

Murugaiyan, Mittal, Weiner: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).

Target Details for IL-27

Target: IL-27 (IL27) (Interleukin 27 (IL27))

Alternative Name: Il27 (IL27 Products)

Background: Background: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.

Gene ID: 246779

NCBI Accession: NP_663611

UniProt: Q8K3I6