Myc Tag antibody

Catalog No. ABIN104383

Product Details anti-Myc Tag Antibody

Target: Myc Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myc Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Sequence: EQKLISEEDL

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide.  The sequence corresponds to amino acids 410-419 of human c-Myc.

Isotype: IgG

Application Details

Application Notes: Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene.   As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope.  The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins.  This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Myc Tag antibody (ABIN104383)

Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

Fujikawa, Matsumoto, Kuboyama, Suzuki, Noda: "Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5." in: PLoS ONE, Vol. 10, Issue 3, pp. e0119361, (2016) (PubMed).

Sorensen, Conner: "γ-secretase-dependent cleavage initiates notch signaling from the plasma membrane." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 9, pp. 1234-45, (2011) (PubMed).

Target Details for Myc Tag

Target: Myc Tag

Abstract: Myc Tag Products

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein.  Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.