IL17F antibody (HRP)

Catalog No. ABIN1043827

Product Details anti-IL17F Antibody (HRP)

Target: IL17F (Interleukin 17F (IL17F))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL17F antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Specificity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Characteristics: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Purification: purified

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: Il17f

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide!

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-IL17F antibody (HRP) (ABIN1043827)

Gomez-Rodriguez, Sahu, Handon, Davidson, Anderson, Kirby, August, Schwartzberg: "Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase." in: Immunity, Vol. 31, Issue 4, pp. 587-97, (2009) (PubMed).

Target Details for IL17F

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: IL-17F (IL17F Products)

Synonyms: CANDF6 antibody, IL-17F antibody, ML-1 antibody, ML1 antibody, C87042 antibody, IL17F antibody, interleukin 17F antibody, IL17F antibody, Il17f antibody

Background: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Synonyms: IL-17F, Interleukin-17F, ML1, ML-1, IL17F, and IL24

Gene ID: 257630

NCBI Accession: NP_665855

UniProt: Q7TNI7

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity