IDO2 antibody

Catalog No. ABIN1043821

Supplier Product No.: 210-301-e59

Product Details anti-IDO2 Antibody

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Supplier Product No.: 210-301-e59

Supplier: Rockland

Purpose: IDO2 Antibody

Cross-Reactivity (Details): IDO2 antibody is specific for mouse IDO2 protein.

Characteristics: Synonyms: mouse anti-IDO2 Antibody, Indoleamine 2,3-dioxygenase 2, Indol1, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1, Ido-2, Ido 2, IDO2 antibody, mouse anti-IDO-2 antibody

Purification: Anti-IDO2 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 1HC

Isotype: IgG2a kappa

Application Details

Application Notes:

Application Note: Anti-IDO2 antibody has been tested in western blot and is suitable for use in ELISA and IF. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

ELISA Dilution: 1:5000 - 1:50000

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IDO2 antibody (ABIN1043821)

Wolff, Driever, Erdlenbruch, Kortmann, Rutkowski, Pietsch, Parker, Metz, Gnekow, Kramm: "Intensive chemotherapy improves survival in pediatric high-grade glioma after gross total resection: results of the HIT-GBM-C protocol." in: Cancer, Vol. 116, Issue 3, pp. 705-12, (2010) (PubMed).

Metz, Duhadaway, Rust, Munn, Muller, Mautino, Prendergast: "Zinc protoporphyrin IX stimulates tumor immunity by disrupting the immunosuppressive enzyme indoleamine 2,3-dioxygenase." in: Molecular cancer therapeutics, Vol. 9, Issue 6, pp. 1864-71, (2010) (PubMed).

Target Details for IDO2

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Alternative Name: Ido2 (IDO2 Products)

Background: Background: Indoleamine 2,3-dioxygenase 2 (IDO2) is related to IDO1, and may play a role in T and B Cell Immunity. IDO2's role in immune regulation will be become clearer following the completion of genetic knock-out studies. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.

Gene ID: 209176

NCBI Accession: NP_666061

UniProt: Q8R0V5