IDO1 antibody

Catalog No. ABIN1043819

Supplier Product No.: 210-301-e58

Product Details anti-IDO1 Antibody

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Supplier Product No.: 210-301-e58

Supplier: Rockland

Purpose: IDO1 Antibody

Cross-Reactivity (Details): IDO1 antibody is specific for mouse IDO1 protein.

Characteristics: Synonyms: mouse anti-IDO1 antibody, Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido 1, Ido-1, IDO1 antibody, mouse anti-IDO-1 antibody

Purification: Anti-IDO1 antibody was purified from ascites fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: IDO1 antibody was produced in mouse by repeated immunizations with mouse recombinant IDO1 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 2E2-6

Isotype: IgG1

Application Details

Application Notes:

Flow Cytometry Dilution: 0.5-1x10^6 cells

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IF, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

Immunoprecipitation Dilution: 10-100 μL

ELISA Dilution: 1:5000-1:50000

IF Microscopy Dilution: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IDO1 antibody (ABIN1043819)

Mellor, Munn: "IDO expression by dendritic cells: tolerance and tryptophan catabolism." in: Nature reviews. Immunology, Vol. 4, Issue 10, pp. 762-74, (2004) (PubMed).

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: Ido1 (IDO1 Products)

Background: Background: Anti-IDO-1 antibody recognizes indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Gene ID: 15930

NCBI Accession: NP_032350

UniProt: P28776

Pathways: Activated T Cell Proliferation