IL-9 antibody (Biotin)

Catalog No. ABIN1043810

Product Details anti-IL-9 Antibody (Biotin)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-9 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: IL-9 Antibody Biotin Conjugated

Characteristics: Synonyms: rabbit anti-IL-9 biotin conjugated antibody, rabbit anti-interleukin-9 biotin conjugated antibody, Interleukin 9, IL 9, IL9, T cell growth factor 3, T cell growth factor p40, TCGF 3, Mast cell growth factor, MCGF, Megakaryoblast growth factor, p40 cytokine

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-9 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL-9 antibody (Biotin) (ABIN1043810)

Zhu, Liu, Zhao, Jia, Cui, Ning: "Association analysis of polymorphisms in IL-3, IL-4, IL-5, IL-9, and IL-13 with Graves' disease." in: Journal of endocrinological investigation, Vol. 33, Issue 10, pp. 751-5, (2010) (PubMed).

Ciprandi: "Serum interleukin 9 in allergic rhinitis." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 104, Issue 2, pp. 180-1, (2010) (PubMed).

Osterfeld, Ahrens, Strait, Finkelman, Renauld, Hogan: "Differential roles for the IL-9/IL-9 receptor alpha-chain pathway in systemic and oral antigen-induced anaphylaxis." in: The Journal of allergy and clinical immunology, Vol. 125, Issue 2, pp. 469-476.e2, (2010) (PubMed).

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: IL9 (IL9 Products)

Background: Background: IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Gene ID: 3578

NCBI Accession: NP_000581

UniProt: P15248

Pathways: JAK-STAT Signaling