GDF15 antibody (Biotin)

Catalog No. ABIN1043807

Supplier Product No.: 209-406-b88

Product Details anti-GDF15 Antibody (Biotin)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF15 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 209-406-b88

Supplier: Rockland

Purpose: GDF15 Antibody Biotin Conjugated

Cross-Reactivity (Details): This antibody reacts with endogenous NAG-1 protein from human tissues. A BLAST analysis using the immunizing recombinant protein suggests reactivity with NAG-1 from human and chimpanzee based on 100 % and 98 % homology, respectively, and with macaque based on an 94 % homology, and with mouse and rat based on 70 % homology with the immunizing sequence.

Characteristics: Synonyms: rabbit anti-NAG-1 biotin Conjugated Antibody, NAG1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Immunogen:

Immunogen: This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein.  Cross reactivity to MBP was removed via cross-adsorption chromatography.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This Protein-A purified, MBP-cross-adsorbed NAG1/GDF15 Biotin conjugated antibody has ben tested by Western blot of human and mouse NAG-1 protein and is suitable for ELISA.   For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG1/GDF15 C-terminal specific antibodies.  This antibody is useful in dual antibody immunometric assays (EIA). Specific conditions for reactivity should be optimized by the end user.  Expect bands in western blots of approximately 14 kDa in size corresponding to NAG-1 monomer using the appropriate cell lysate or extract.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-GDF15 antibody (Biotin) (ABIN1043807)

Baek, Eling: "Changes in gene expression contribute to cancer prevention by COX inhibitors." in: Progress in lipid research, Vol. 45, Issue 1, pp. 1-16, (2006) (PubMed).

Target Details for GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Alternative Name: GDF15 (GDF15 Products)

Background: Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Gene ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interactome