IL32 antibody (Biotin)

Catalog No. ABIN1043804

Product Details anti-IL32 Antibody (Biotin)

Target: IL32 (Interleukin 32 (IL32))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL32 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: IL-32A Antibody Biotin Conjugated

Cross-Reactivity (Details): In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids.

Characteristics: Synonyms: rabbit anti-IL-32A biotin conjugated antibody, rabbit anti-interleukin-32A biotin conjugated antibody, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32, IL-32 isoform 4

Purification: This purified antibody has been heated to 56°C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to native human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000-1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL32 antibody (Biotin) (ABIN1043804)

Nishida, Andoh, Inatomi, Fujiyama: "Interleukin-32 expression in the pancreas." in: The Journal of biological chemistry, Vol. 284, Issue 26, pp. 17868-76, (2009) (PubMed).

Target Details for IL32

Target: IL32 (Interleukin 32 (IL32))

Alternative Name: IL32 (IL32 Products)

Background: Background: IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFα and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Gene ID: 9235, 61658634

UniProt: P24001