IL-3 antibody (HRP)

Catalog No. ABIN1043802

Supplier Product No.: 209-403-b92

Product Details anti-IL-3 Antibody (HRP)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 209-403-b92

Supplier: Rockland

Purpose: IL-3 Antibody Peroxidase Conjugated

Characteristics: Synonyms: rabbit anti-IL-3 peroxidase antibody, rabbit anti-Interleukin 3 peroxidase antibody, IL3, interleukin-3, Burst promoting activity, Colony stimulating factor, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL-3, Hematopoietic growth factor, Mast cell growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor

Purification: Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogen:

Immunogen: Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500-1:2,500

Application Note: This IL-3 antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000-1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Human IL-3 (RABBIT) Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL-3 antibody (HRP) (ABIN1043802)

Davila, Froeling, Tan, Bonnard, Boland, Snippe, Hibberd, Seielstad: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, (2010) (PubMed).

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3 (IL-3 Products)

Background: Background: The pleiotropic IL3 (Interleukin 3) is a 15 kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis.

Gene ID: 3562

NCBI Accession: NP_000579

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy