CCL20 antibody (Biotin)

Catalog No. ABIN1043793

Product Details anti-CCL20 Antibody (Biotin)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL20 antibody is conjugated to Biotin

Application: Western Blotting (WB)

Specificity: This product was purified from mouse ascites by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human MIP-3alpha protein. A BLAST analysis was used to suggest cross-reactivity with MIP-3alpha from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with MIP-3alpha from other sources has not been determined.

Characteristics: MIP-3alpha (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3alpha inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3alpha also possesses antibacterial activity against E.coli and S.aureus. MIP-3alpha is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Purification: purified

Immunogen: Anti-MIP-3alpha (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human MIP-3alpha produced in E.coli followed by hybridoma development.
Immunogen Type: RecombinantProtein

Clone: 3N2D9

Isotype: IgG

Application Details

Application Notes: This purified antibody has been tested for use in Western Blot. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: CCL20

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-CCL20 antibody (Biotin) (ABIN1043793)

Lisignoli, Manferdini, Codeluppi, Piacentini, Grassi, Cattini, Filardo, Facchini: "CCL20/CCR6 chemokine/receptor expression in bone tissue from osteoarthritis and rheumatoid arthritis patients: different response of osteoblasts in the two groups." in: Journal of cellular physiology, Vol. 221, Issue 1, pp. 154-60, (2009) (PubMed).

Target Details for CCL20

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Alternative Name: MIP-3alpha (CCL20 Products)

Synonyms: MIP-3ALPHA antibody, CCL20 antibody, si:dkey-150o13.1 antibody, CKb4 antibody, LARC antibody, MIP-3-alpha antibody, MIP-3a antibody, MIP3A antibody, SCYA20 antibody, ST38 antibody, MIP-3A antibody, MIP-3[a] antibody, Scya20 antibody, exodus-1 antibody, C-C motif chemokine ligand 20 antibody, chemokine (C-C motif) ligand 20b antibody, chemokine (C-C motif) ligand 20 antibody, CCL20 antibody, ccl20b antibody, Ccl20 antibody

Background: MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Synonyms: MIP-3α , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1

Gene ID: 6364, 4759076

UniProt: P78556

Pathways: The Global Phosphorylation Landscape of SARS-CoV-2 Infection