IL-25 antibody

Catalog No. ABIN1043788

Product Details anti-IL-25 Antibody

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: IL-17E Antibody

Cross-Reactivity (Details): This antibody is specific for human IL-17E protein.

Characteristics: Synonyms: mouse Anti-IL-17E Antibody, mouse anti-IL-25 antibody, Interleukin 25, IL-25, Interleukin 17E, IL-17E

Purification: Anti-IL-17E was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 11K8B1

Isotype: IgG1

Application Details

Application Notes:

Flow Cytometry Dilution: 1 μg/mL

Application Note: IL 17E antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.5 μg/mL

ELISA Dilution: 1:10,000-1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IL-25 antibody (ABIN1043788)

Conti, Shen, Nayyar, Stocum, Sun, Lindemann, Ho, Hai, Yu, Jung, Filler, Masso-Welch, Edgerton, Gaffen: "Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis." in: The Journal of experimental medicine, Vol. 206, Issue 2, pp. 299-311, (2009) (PubMed).

Target Details for IL-25

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Alternative Name: IL25 (IL25 Products)

Background: Background: Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.

Gene ID: 64806

NCBI Accession: NP_073626

UniProt: Q9H293