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AKT2 antibody (Internal Region)

Rockland 200-501-e71 AKT2 Reactivity: Human WB, ELISA, IHC, FACS, IP Host: Rat Monoclonal 16G11-E8 unconjugated
Rockland
Catalog No. ABIN1043774
Supplier Product No.: 200-501-e71
  • Target See all AKT2 Antibodies
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Binding Specificity
    • 21
    • 17
    • 15
    • 15
    • 15
    • 14
    • 8
    • 7
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 162
    • 94
    • 58
    • 11
    • 8
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Human
    Host
    • 148
    • 24
    • 2
    • 2
    • 1
    • 1
    Rat
    Clonality
    • 150
    • 26
    Monoclonal
    Conjugate
    • 93
    • 12
    • 11
    • 10
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This AKT2 antibody is un-conjugated
    Application
    • 148
    • 84
    • 61
    • 31
    • 28
    • 26
    • 26
    • 17
    • 16
    • 10
    • 7
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
    Supplier Product No.
    200-501-e71
    Supplier
    Rockland
    Purpose
    AKT2 Antibody
    Cross-Reactivity (Details)
    Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein.
    Characteristics
    Synonyms: rat anti-Anti-AKT2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

    Immunogen Type: Conjugated Peptide

    Clone
    16G11-E8
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product AKT2 Primary Antibody
  • Application Notes

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: 20 μg/mL

    Application Note: Anti-AKT2 Antibody is tested for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT2 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

    Western Blot Dilution: 1:500- 1:2000

    Immunoprecipitation Dilution: 20 μg/mL

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Altomare, Kozak, Sonoda, Testa: "Chromosome mapping of the mouse Akt2 gene and Akt2 pseudogene." in: Cytogenetics and cell genetics, Vol. 74, Issue 4, pp. 248-51, (1997) (PubMed).

  • Target
    AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
    Alternative Name
    AKT2 (AKT2 Products)
    Background
    Background: AKT2 Antibody detects AKT2 which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT2 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
    Gene ID
    208
    NCBI Accession
    NP_001317440
    UniProt
    P31751
    Pathways
    PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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