BIN3 antibody

Catalog No. ABIN1043741

Product Details anti-BIN3 Antibody

Target: BIN3 (Bridging Integrator 3 (BIN3))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: BIN3 Antibody

Cross-Reactivity (Details): BIN3 antibody is specific for human BIN3 protein.

Characteristics: Synonyms: mouse anti-BIN3 Antibody, Bridging integrator 3, BIN-3, BIN 3, BIN3 antibody, anti-BIN-3 antibody

Purification: Anti-BIN3 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 3A4

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-BIN3 antibody has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1500

Immunoprecipitation Dilution: 10-100 μL

ELISA Dilution: 1:5000-1:50000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-BIN3 antibody (ABIN1043741)

Routhier, Burn, Abbaszade, Summers, Albright, Prendergast: "Human BIN3 complements the F-actin localization defects caused by loss of Hob3p, the fission yeast homolog of Rvs161p." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21670-7, (2001) (PubMed).

Target Details for BIN3

Target: BIN3 (Bridging Integrator 3 (BIN3))

Alternative Name: BIN3 (BIN3 Products)

Background: Background: BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non-Hodgkin's lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.

Gene ID: 55909

NCBI Accession: NP_061158

UniProt: Q9NQY0