BIN1 antibody

Catalog No. ABIN1043735

Product Details anti-BIN1 Antibody

Target: BIN1 (Bridging Integrator 1 (BIN1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: Anti-BIN1 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above. BIN1 antibody is specific for human BIN1 protein. A BLAST analysis was used to suggest cross-reactivity with BIN1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with BIN1 from other sources has not been determined.

Characteristics: Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive, also known as autosomal recessive myotubular myopathy.

Sterility: Sterile filtered

Immunogen: Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Clone: 99D

Isotype: IgG2b

Application Details

Application Notes: Anti-BIN1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: BIN1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-BIN1 antibody (ABIN1043735)

Nicot, Toussaint, Tosch, Kretz, Wallgren-Pettersson, Iwarsson, Kingston, Garnier, Biancalana, Oldfors, Mandel, Laporte: "Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy." in: Nature genetics, Vol. 39, Issue 9, pp. 1134-9, (2007) (PubMed).

Sinha-Datta, Datta, Ghorbel, Dodon, Nicot: "Human T-cell lymphotrophic virus type I rex and p30 interactions govern the switch between virus latency and replication." in: The Journal of biological chemistry, Vol. 282, Issue 19, pp. 14608-15, (2007) (PubMed).

Target Details for BIN1

Target: BIN1 (Bridging Integrator 1 (BIN1))

Alternative Name: BIN1 (BIN1 Products)

Synonyms: bin1 antibody, MGC53185 antibody, amph2 antibody, amphl antibody, sh3p9 antibody, MGC76187 antibody, cb57 antibody, zgc:86701 antibody, BIN1 antibody, AMPH2 antibody, AMPHL antibody, SH3P9 antibody, ALP-1 antibody, Amphl antibody, BRAMP-2 antibody, bridging integrator 1 antibody, bridging integrator 1 S homeolog antibody, bridging integrator 1b antibody, BIN1 antibody, bin1.S antibody, bin1 antibody, bin1b antibody, Bin1 antibody

Background: Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.
Synonyms: AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN-1, BIN 1, BIN1 antibody

Gene ID: 274

NCBI Accession: NP_004296

UniProt: O00499