IDO1 antibody

Catalog No. ABIN1043733

Product Details anti-IDO1 Antibody

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Specificity: Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.

Characteristics: Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.

Sterility: Sterile filtered

Immunogen: Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein

Clone: 10-1

Isotype: IgG3

Application Details

Application Notes: Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.

Comment:

Gene Name: Ido1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References for anti-IDO1 antibody (ABIN1043733)

Meyer, Klatte, Dinh, Harries, Reithmayer, Meyer, Sinclair, Paus: "Evidence that the bulge region is a site of relative immune privilege in human hair follicles." in: The British journal of dermatology, Vol. 159, Issue 5, pp. 1077-85, (2009) (PubMed).

Batista, Juhász, Muzik, Kupsky, Barger, Chugani, Mittal, Sood, Chakraborty, Chugani: "Imaging correlates of differential expression of indoleamine 2,3-dioxygenase in human brain tumors." in: Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, Vol. 11, Issue 6, pp. 460-6, (2009) (PubMed).

Zhu, Ji, Wang, Zhang, Liu, Zhang, Matsushima, Cao, Zhang: "Synovial autoreactive T cells in rheumatoid arthritis resist IDO-mediated inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 11, pp. 8226-33, (2007) (PubMed).

Mellor, Munn: "IDO expression by dendritic cells: tolerance and tryptophan catabolism." in: Nature reviews. Immunology, Vol. 4, Issue 10, pp. 762-74, (2004) (PubMed).

Target Details for IDO1

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO1 (IDO1 Products)

Synonyms: INDO antibody, ido antibody, indo antibody, IDO antibody, cd107b antibody, IDO1 antibody, IDO-1 antibody, Ido antibody, Indo antibody, indoleamine 2,3-dioxygenase 1 antibody, indoleamine 2,3-dioxygenase 1 L homeolog antibody, indoleamine 2,3-dioxygenase 1-like antibody, IDO1 antibody, ido1.L antibody, ido1 antibody, Ido1 antibody, LOC100050681 antibody

Background: Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Synonyms: Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1

Gene ID: 3620

NCBI Accession: NP_002155

UniProt: P14902

Pathways: Activated T Cell Proliferation