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- Target See all Biotin products
- Biotin
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Biotin antibody is un-conjugated
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Application
- Immunoprecipitation (IP), Conjugation (Con)
- Specificity
- Anti-Biotin Antibody was prepared from mouse ascites by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
- Characteristics
- Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
- Sterility
- Sterile filtered
- Immunogen
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Anti-Biotin Antibody was produced by repeated immunizations withBiotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
Immunogen Type: Other - Isotype
- IgG1
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anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated
anti-Biotin antibodyELISA Host: Goat Polyclonal unconjugated Fab fragment
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibody (DyLight 488)WB, FM, FLISA Host: Goat Polyclonal DyLight 488
anti-Biotin antibodyFACS, ELISA Host: Mouse Monoclonal SB58c unconjugated
anti-Biotin antibodyFACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated
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- Application Notes
- Anti-Biotin antibody is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
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- Target
- Biotin
- Abstract
- Biotin Products
- Target Type
- Chemical
- Background
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Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
Synonyms: Vitamin H, Coenzyme R
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