Biotin antibody

Catalog No. ABIN1043715

Supplier Product No.: 200-301-098

Product Details anti-Biotin Antibody

Target: Biotin

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Biotin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-098

Supplier: Rockland

Purpose: Biotin Monoclonal Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Characteristics: Synonyms: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R

Purification: Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).

Immunogen Type: Other

Clone: 4C7-D5

Isotype: IgG2a lambda

Application Details

Application Notes:

Flow Cytometry Dilution: 1-2 μg/mL

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Biotin antibody has been tested in ELISA, western blot and immunofluorescence and is suitable for lateral flow, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000

IF Microscopy Dilution: 2 μg/mL

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Biotin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.