Fluorescein antibody

Catalog No. ABIN1043713

Product Details

Target: Fluorescein

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Specificity: FLUORESCEIN Antibody was prepared from mouse ascites by Protein A/G chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.

Characteristics: Anti Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Sterility: Sterile filtered

Immunogen: Fluorescein conjugated to Mouse IgG
Immunogen Type: Other

Isotype: IgG1

Application Details

Application Notes: Fluorescein Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Expiry Date: 12 months

References

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Noga, Udomkusonsri: "Fluorescein: a rapid, sensitive, nonlethal method for detecting skin ulceration in fish." in: Veterinary pathology, Vol. 39, Issue 6, pp. 726-31, (2002) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Anti Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC