aHSP antibody

Catalog No. ABIN1043695

Product Details anti-aHSP Antibody

Target: aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This aHSP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: AHSP Antibody

Cross-Reactivity (Details): Anti-AHSP Antibody is directed against the human AHSP protein.

Characteristics: Synonyms: rabbit anti-AHSP antibody, Alpha-hemoglobin-stabilizing protein (AHSP), Erythroid differentiation-related factor, Erythroid-associated factor, EDRF, ERAF

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen:

Immunogen: Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.

Immunogen Type: Recombinant Protein

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:8000

Application Note: Anti-AHSP Antibody has been tested in western blot and immunohistochemistry and is suitable for immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 86 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-aHSP antibody (ABIN1043695)

Nasimuzzaman, Khandros, Wang, Kong, Zhao, Weiss, Rivella, Weiss, Persons: "Analysis of alpha hemoglobin stabilizing protein overexpression in murine β-thalassemia." in: American journal of hematology, Vol. 85, Issue 10, pp. 820-2, (2010) (PubMed).

Target Details for aHSP

Target: aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

Alternative Name: AHSP (aHSP Products)

Background: Background: AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts.

Gene ID: 51327

NCBI Accession: NP_057717

UniProt: Q9NZD4