SLC12A4
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
Immunoblotting: use at 3 μg/mL. A band of ~140 kDa is detected. Immunohistochemistry: use at 1-5 μg/mL. Positive control: Adult rat brain These are recommended concentrations, Enduser should determine optimal concentrations for their applications.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Dilute in PBS or medium which is identical to that used in the assay system.
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
This antibody is stable for at least one (1) year at -20°C.
Target
SLC12A4
(Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))
KCC1 antibody, kcc1 antibody, SLC12A4 antibody, AW546649 antibody, RBCKCC1 antibody, Kcc1 antibody, solute carrier family 12 member 4 antibody, solute carrier family 12 (potassium/chloride transporter), member 4 L homeolog antibody, solute carrier family 12, member 4 antibody, SLC12A4 antibody, slc12a4.L antibody, Slc12a4 antibody
Background
Solute carrier family 12 member 5,Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of dendritic spine formation and maturation (PubMed:22345354). {PubMed:22345354, PubMed:9930699}.,KCC2 is a member of the cation-chloride cotransporter gene family. KCCs lower intracellular chloride concentrations below the electrochemical equilibrium potential. Depending on the concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. KCC2 is expressed at high levels in neurons throughout the nervous system and is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling resulting in motor defects, epilepsy, and anxiety-like behavior.,Membrane, Multi-pass membrane protein.,Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter