Histone H4 antibody (meLys20)
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- Target See all Histone H4 Antibodies
- Histone H4
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Binding Specificity
- meLys20
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Histone H4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
- Specificity
- Specific for K20 mono-methylated histone H4, no cross-reactivity with K20 tri-, di-, or unmethylated histone H4, no cross-reactivity with histone H3.
- Cross-Reactivity
- Arabidopsis thaliana, Drosophila melanogaster, Mammalian, Xenopus laevis
- Cross-Reactivity (Details)
- Human. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals
- Purification
- Protein G
- Immunogen
- Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched))
- Clone
- 5E10-D8
- Isotype
- IgG1
- Top Product
- Discover our top product Histone H4 Primary Antibody
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- Application Notes
- Optimal antibody dilution should be determined by titration, however as a guideline try, WB: Use a concentration of 1 μg/mL. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).Can be blocked with Histone H4 peptide - mono methyl K20
- Comment
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Myeloma, fusion partners: X63-Ag8.653
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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