CD34 antibody (Extracellular Domain)
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- Target See all CD34 Antibodies
- CD34
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Binding Specificity
- Extracellular Domain
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Reactivity
- Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD34 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Cell-ELISA (cELISA)
- Characteristics
- Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
- Purification
- Protein G
- Immunogen
- genetic immunisation with cDNA encoding the extracellular domain of ovine CD34
- Clone
- EQ-8D11-C1
- Isotype
- IgG2a
- Top Product
- Discover our top product CD34 Primary Antibody
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- Application Notes
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Flow cytometry: 1.2 µg/10^6 cells
Immunofluorescence: 1 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration. - Restrictions
- For Research Use only
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- Buffer
- PBS, pH 7.2
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C
- Storage Comment
- short term: 2 °C - 8 °C, long term: -20 °C
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- Target
- CD34
- Alternative Name
- Ovine CD34 (CD34 Products)
- Background
- CD34 is a type I membrane protein with an apparent MW of approximately 115 kDa. It is a putative adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. It is thought to act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components.
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