CD51 antibody

Catalog No. ABIN1042463

Product Details anti-CD51 Antibody

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: T-lymphocyte clones NKI-M7 detect a melanoma-associated proteoglycan (MW greater than 450-250 kDa) and a molecular complex, which under reducing conditions consists of 4 compounds of 120, 95, 29 and 25 kDa respectively. NKI-M7 reacts with cultured melanomas melanomas in frozen sections. The antigen detected by NKI-M7 is strongly associated with the adhesion and cytoplasmic spreading of melanoma cells to plastic surfaces and monolayers of vascular endothelial cells. NKI-M7 blocks fibronectin-induced chemotaxic motility and chemokinesis of melanoma cells. In addition to their membrane localization, the antigens detected by NKI-M7are abundant in extracellular adhesion plaques deposited by cultured melanoma cells. NKI-M7 stains primary tumors heterogeneously, whereas metastases are homogeneously stained.

Characteristics: CD markers,Cell adhesion

Purification: Purified

Clone: NKI-M7 (former AMF7)

Isotype: IgG1

Application Details

Application Notes: NKI-M7 is useful for ELISA, immunoprecipitation, flow cytometry and immunohistochemsitry on frozen sections. Optimal antibody dilution should be determined by titration.

Comment:

NKI-M7 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cultured melanoma cells derived from a human melanoma metastasis.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.09% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD51 antibody (ABIN1042463)

Atanes, Gómez, Aspe, de Toro, Graña, Sánchez, Galdo: "[Erythema nodosum: a study of 160 cases]." in: Medicina clínica, Vol. 96, Issue 5, pp. 169-72, (1991) (PubMed).

Quinn, Athanasou, McGee: "Extracellular matrix receptor and platelet antigens on osteoclasts and foreign body giant cells." in: Histochemistry, Vol. 96, Issue 2, pp. 169-76, (1991) (PubMed).

de Vries, Keizer, te Velde, Voordouw, Ruiter, Rümke, Spits, Figdor: "Characterization of melanoma-associated surface antigens involved in the adhesion and motility of human melanoma cells." in: International journal of cancer. Journal international du cancer, Vol. 38, Issue 4, pp. 465-73, (1986) (PubMed).

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: vitronectin receptor CD51 (ITGAV Products)

Synonyms: 1110004F14Rik antibody, 2610028E01Rik antibody, CD51 antibody, D430040G12Rik antibody, MSK8 antibody, VNRA antibody, VTNR antibody, Cd51 antibody, integrin subunit alpha V antibody, integrin alpha V antibody, ITGAV antibody, Itgav antibody

Background: CD51 (Cluster of Differentiation 51), known as vitronectin receptor alpha chain, or integrin alphaV is a type I integral membrane glycoprotein. CD51 forms a heterodimer with integrin beta1 (CD29), beta3 (CD61), beta5, beta6, or beta8. CD51 contains two disulfide-linked subunits of 125 kD and 24 kD. Many extracellular matrix proteins with RGD-motifs are CD51 ligands. In association with its beta chains, CD51 binds vitronectin, von Willebrand factor, fibronectin, thrombospondin, osteopontin, fibrinogen, and laminin. CD51, as an adhesion molecule, plays important roles in leukocytes homing and rolling, mediates bone absorption and angiogenesis. CD51 is expressed on endothelial cells, fibroblasts, macrophages, platelets, osteoclasts, neuroblastoma, melanoma, and hepatoma cells.

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex