Merlin antibody (pSer518)

Catalog No. ABIN104130

Product Details anti-Merlin Antibody

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Binding Specificity: pSer518

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Merlin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: NF2 phospho S518 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product).

Characteristics: Synonyms: rabbit anti-NF2 pS518 antibody, rabbit anti-Merlin pS518 antibody, moesin ezrin radixin like protein antibody, Neurofibromatosis 2 antibody, Neurofibromin-2, Schwannomerlin, Schwannomin, Merlin, NF 2, NF-2, SCH

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA.  By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Immunoblotting will detect human and mouse NF2 (Merlin).  Although not tested, this antibody is likely functional in Immunofluorescence, immunohistochemistry, and immunoprecipitation.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:1,200,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Merlin antibody (ABIN104130)

Hu, Du, Shelton, Oldham, DiPersio, Klein: "An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice." in: Cell stem cell, Vol. 21, Issue 1, pp. 91-106.e6, (2018) (PubMed).

Angelo, Wu, Meng, Sun, Kopetz, McCutcheon, Slopis, Kurzrock: "Combining Curcumin (Diferuloylmethane) and Heat Shock Protein Inhibition for Neurofibromatosis 2 Treatment: Analysis of Response and Resistance Pathways." in: Molecular cancer therapeutics, (2011) (PubMed).

Lallemand, Saint-Amaux, Giovannini: "Tumor-suppression functions of merlin are independent of its role as an organizer of the actin cytoskeleton in Schwann cells." in: Journal of cell science, Vol. 122, Issue Pt 22, pp. 4141-9, (2010) (PubMed).

Wang, Lu, Tang, Wang, Wu: "The phosphorylation status of merlin in sporadic vestibular Schwannomas." in: Molecular and cellular biochemistry, Vol. 324, Issue 1-2, pp. 201-6, (2009) (PubMed).

Target Details for Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Alternative Name: NF2 (NF2 Products)

Background: Background: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin).  The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM's suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane.  The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa.  This antibody is specific for the phosphorylated form of human NF2 (Merlin).

Gene ID: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization