MDH1 antibody

Catalog No. ABIN103904

Product Details anti-MDH1 Antibody

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This MDH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: Malate Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Characteristics: Synonyms: sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase

Purification: Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: MALATE DEHYDROGENASE antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Alternative Name: MDH1 (MDH1 Products)

Background: Background: Catalytic activity of Malate Dehydrogenase is (S)-malate + NAD+ = oxaloacetate + NADH.

Gene ID: 396894

UniProt: P11708