MAD2L2 antibody (N-Term)

Catalog No. ABIN103894

Product Details anti-MAD2L2 Antibody

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MAD2L2 Antibody

Characteristics: Synonyms: rabbit anti-MAD2L2 antibody, MAD2B, REV7, hREV7, REV homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human MAD2L2. 

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  For western blotting HeLa whole cell lysates and nuclear extracts are suggested.  Reactivity in other immunoassays is unknown.  Antibody activity can be blocked using a control peptide (000-001-470).

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:7,500 - 1:30,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.68 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAD2L2

Target: MAD2L2 (MAD2 Mitotic Arrest Deficient-Like 2 (MAD2L2))

Alternative Name: MAD2L2 (MAD2L2 Products)

Background: Background: MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein. MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.

Gene ID: 187960072

UniProt: Q9UI95