Luciferase antibody

Catalog No. ABIN103637

Supplier Product No.: 100-101-150

Product Details anti-Luciferase Antibody

Target: Luciferase

Reactivity: Photinus pyralis

Host: Goat

Clonality: Polyclonal

Conjugate: This Luciferase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-101-150

Supplier: Rockland

Purpose: Luciferase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Luciferase [Firefly].

Characteristics: Synonyms: Goat Anti-Luciferase Antibody, Luciferase Firefly, Goat Anti Luciferase

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen:

Immunogen: Luciferase [Firefly]

Immunogen Type: Native Protein

Application Details

Application Notes:

Application Note: Anti-Luciferase Antibody has been tested by western blot and is suitable in ELISA. This product can be assayed against 1.0 μg of Luciferase [Firefly] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References for anti-Luciferase antibody (ABIN103637)

Windahl, Lagerquist, Andersson, Jochems, Kallkopf, Håkansson, Inzunza, Gustafsson, van der Saag, Carlsten, Pettersson, Ohlsson: "Identification of target cells for the genomic effects of estrogens in bone." in: Endocrinology, Vol. 148, Issue 12, pp. 5688-95, (2008) (PubMed).

Kenny, Duval, Sammut, Steele, Pritchard, Atherton, Argent, Dimaline, Dockray, Varro: "Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).

Target Details for Luciferase

Target: Luciferase

Abstract: Luciferase Products

Background: Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.