Left-Right Determination Factor (LEFTY-A) antibody

Catalog No. ABIN103459

Product Details

Target: Left-Right Determination Factor (LEFTY-A)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Immunogentype:Recombinant

Clone: 7C5G1H6H10

Isotype: IgG

Application Details

Application Notes: This antibody is suitable for ELISA and western blotting.  The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Ulloa, Tabibzadeh: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, (2001) (PubMed).

Tsukui, Capdevila, Tamura, Ruiz-Lozano, Rodriguez-Esteban, Yonei-Tamura, Magallón, Chandraratna, Chien, Blumberg, Evans, Belmonte: "Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11376-81, (1999) (PubMed).

Meno, Shimono, Saijoh, Yashiro, Mochida, Ohishi, Noji, Kondoh, Hamada: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal." in: Cell, Vol. 94, Issue 3, pp. 287-97, (1998) (PubMed).

Target Details

Target: Left-Right Determination Factor (LEFTY-A)

Alternative Name: LEFTY A (LEFTY-A Products)

Background: During vertebrate embryogenesis, a left-right axis is established.  Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries.  TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors.   Complexes formed move into the nucleus, where they act as components of a transcriptional complex.  Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling.  Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor.  Lefty also inhibits events downstream from R-Smad phosphorylation.  Lefty provides a repressed state of TGF-beta-responsive genes.  The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.  Members of the TGF-beta superfamily require processing for their activation.   Cleavage is therefore an essential step for Lefty activation.  Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.
Synonyms: Left-right determination factor 2 antibody, Protein lefty-2 antibody, Left-right determination factor A antibody, Protein lefty-A antibody, Transforming growth factor beta-4 antibody, TGF-beta-4 antibody,Endometrial bleeding-associated factor antibody

Gene ID: 320202

UniProt: P57785, O00292