LEFTY2 antibody

Catalog No. ABIN103459

Supplier Product No.: 200-301-279

Product Details anti-LEFTY2 Antibody

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEFTY2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-301-279

Supplier: Rockland

Purpose: LEFTY A Antibody

Cross-Reactivity (Details): No reactivity occurs against 6X HIS tag.

Characteristics: Synonyms: mouse anti-leftyA antibody, mouse anti-protein lefty 2 antibody, Left-right determination factor 2 antibody, Protein lefty-2 antibody, Left-right determination factor A antibody, Protein lefty-A antibody, Transforming growth factor beta-4 antibody, TGF-beta-4 antibody, Endometrial bleeding-associated factor antibody

Purification: This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY.

Sterility: Sterile filtered

Immunogen:

Immunogen: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

Immunogen Type: Recombinant Protein

Clone: 7C5G1H6H10

Isotype: IgG2a kappa

Application Details

Application Notes:

Application Note: This antibody has been tested by ELISA and western blotting.  The antibody may be used for other applications, such as RIA, immunohistochemistry or immunoprecipitation, but specific reaction conditions have not been developed.

Western Blot Dilution: 1:2,000

ELISA Dilution: 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-LEFTY2 antibody (ABIN103459)

Ulloa, Tabibzadeh: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, (2001) (PubMed).

Tsukui, Capdevila, Tamura, Ruiz-Lozano, Rodriguez-Esteban, Yonei-Tamura, Magallón, Chandraratna, Chien, Blumberg, Evans, Belmonte: "Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11376-81, (1999) (PubMed).

Meno, Shimono, Saijoh, Yashiro, Mochida, Ohishi, Noji, Kondoh, Hamada: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal." in: Cell, Vol. 94, Issue 3, pp. 287-97, (1998) (PubMed).

Target Details for LEFTY2

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Alternative Name: LEFTY2 (LEFTY2 Products)

Background: Background: During vertebrate embryogenesis, a left-right axis is established.  Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries.  TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors.   Complexes formed move into the nucleus, where they act as components of a transcriptional complex.  Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling.  Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor.  Lefty also inhibits events downstream from R-Smad phosphorylation.  Lefty provides a repressed state of TGF-beta-responsive genes.  The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans.  Members of the TGF-beta superfamily require processing for their activation.   Cleavage is therefore an essential step for Lefty activation.  Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.

Gene ID: 7044

NCBI Accession: NP_001165896

UniProt: O00292