LIM Domain Binding 1 Protein antibody (C-Term)

Catalog No. ABIN103458

Supplier Product No.: 600-401-350

Product Details anti-LIM Domain Binding 1 Protein Antibody

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 1 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Supplier Product No.: 600-401-350

Supplier: Rockland

Purpose: LDB1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against the mouse LDB1protein.

Characteristics: Synonyms: rabbit anti-LDB1 antibody, rabbit anti-LDB1/CLIM2 antibody, rabbit anti-CLIM2 antibody, LDB-1, CLIM-2, LIM domain-binding protein 1, Carboxyl-terminal LIM domain-binding protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene, a putative LDB1 binding region as suggested by Orkin et al.   Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells.  We suggest using 20 μg for 10E8 cells for ChIP.

ChIP Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:425,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: Ldb1 (LDB1 Products)

Background: Background: LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

Gene ID: 16825, 6754520

UniProt: P70662

Pathways: Stem Cell Maintenance, Chromatin Binding