LIM Domain Binding 1 Protein antibody (AA 361-373)

Catalog No. ABIN103458

Product Details anti-LIM Domain Binding 1 Protein Antibody

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Binding Specificity: AA 361-373

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Binding 1 Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene a putative LDB1 binding region as suggested by Orkin et al.   Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells.  We suggest using 20µg for 10E8 cells for ChIP.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.80 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Alternative Name: LDB1 (LDB1 Products)

Synonyms: CLIM2 antibody, NLI antibody, chi antibody, clim2 antibody, nli antibody, xldb1 antibody, ldb1 antibody, LDB1 antibody, ldb4 antibody, ldb4b antibody, LIM domain binding 1 antibody, LIM domain binding 1 L homeolog antibody, LIM-domain binding 1b antibody, LIM domain binding protein 1 antibody, LIM domain binding 1a antibody, Ldb1 antibody, LDB1 antibody, ldb1.L antibody, ldb1b antibody, xldb1 antibody, ldb1a antibody

Background: LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.
Synonyms: LDB1, CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor.

Gene ID: 16825, 6754520

UniProt: P70662

Pathways: Stem Cell Maintenance, Chromatin Binding