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LPO antibody

Rockland 200-601-146-0100 LPO Reactivity: Cow WB, IP, ELISA Host: Sheep Polyclonal unconjugated
Rockland
Catalog No. ABIN103436
Supplier Product No.: 200-601-146-0100
  • Target See all LPO Antibodies
    LPO (Lactoperoxidase (LPO))
    Reactivity
    • 11
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    Cow
    Host
    • 16
    • 3
    • 2
    Sheep
    Clonality
    • 19
    • 2
    Polyclonal
    Conjugate
    • 11
    • 8
    • 2
    This LPO antibody is un-conjugated
    Application
    • 17
    • 12
    • 10
    • 8
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Supplier Product No.
    200-601-146-0100
    Supplier
    Rockland
    Purpose
    Lactoperoxidase Antibody
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].
    Characteristics
    Synonyms: sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody
    Purification
    Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Lactoperoxidase [Bovine Milk]

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product LPO Primary Antibody
  • Application Notes

    Application Note: Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase [Bovine Milk] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    LPO (Lactoperoxidase (LPO))
    Alternative Name
    Lactoperoxidase (LPO Products)
    Background
    Background: Lactoperoxidase is an antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). It may protect the udder from infection and promote growth in newborn calves. It inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.
    Gene ID
    280844
    UniProt
    P80025
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