This Lactate Dehydrogenase antibody is un-conjugated
Application
Western Blotting (WB), ELISA
Characteristics
Concentration Definition: by UV absorbance at 280 nm
Immunogen
This antibody was prepared from whole goat serum produced by repeated immunizations with a full length lactate dehydrogenase protein isolated from rabbit muscle. Immunogentype:Native
LDH
Reactivity: Lactobacillus plantarum
WB, ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
LACTATE DEHYDROGENASE antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
10.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a ''flipped pattern'') suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M