Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Nuclear Antigen antibody

IPO-38 Reactivity: Mouse IHC, IP, ELISA, WB Host: Mouse Monoclonal IPO-38 unconjugated
Catalog No. ABIN103313
  • Target See all Nuclear Antigen (IPO-38) Antibodies
    Nuclear Antigen (IPO-38)
    Reactivity
    • 5
    • 3
    • 3
    Mouse
    Host
    • 5
    Mouse
    Clonality
    • 5
    Monoclonal
    Conjugate
    • 5
    This Nuclear Antigen antibody is un-conjugated
    Application
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Sterility
    Sterile filtered
    Immunogen
    A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.
    Immunogentype:Native
    Clone
    IPO-38
    Isotype
    IgG
  • Application Notes
    This antibody is suitable for ELISA,  immunoprecipitation, western blotting and immunohistochemistry (frozen and formalin/paraffin).  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsilar tissue is typically used as a positive control.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    Nuclear Antigen (IPO-38)
    Alternative Name
    IPO-38 (IPO-38 Products)
    Background
    The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.
    Synonyms: Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody, HMGN1 antibody, HMG14 antibody
    Gene ID
    15312
    NCBI Accession
    NP_032277
    UniProt
    P18608
You are here:
Support