HMGN1 antibody

Catalog No. ABIN103313

Product Details anti-HMGN1 Antibody

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: IPO-38 Antibody

Cross-Reactivity (Details): This antigen was detected after 12h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes.

Characteristics: Synonyms: mouse anti-IPO38 antibody, mouse anti-HMGN1 antibody, mouse anti-HMG14 antibody, Non-histone chromosomal protein HMG-14 antibody, High-mobility group nucleosome-binding domain-containing protein 1 antibody

Purification: Anti-IPO-38 monoclonal antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Immunogen Type: Native Protein

Clone: IPO-38

Isotype: IgM kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 10 μg/mL

Application Note: Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation.  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsillar tissue is typically used as a positive control.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HMGN1

Target: HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))

Alternative Name: Hmgn1 (HMGN1 Products)

Background: Background: The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.

Gene ID: 15312

NCBI Accession: NP_032277

UniProt: P18608

Pathways: Chromatin Binding