Sucrase Isomaltase, Regulatory (SI-R) antibody (Biotin)
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- Target
- Sucrase Isomaltase, Regulatory (SI-R)
- Reactivity
- Candida sp.
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
- Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-106-189-0100
- Supplier
- Rockland
- Purpose
- Invertase Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Invertase [Candida species].
- Characteristics
- Synonyms: goat anti-Invertase Antibody biotin Conjugated, Biotin Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody
- Purification
- Anti-Invertase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: Invertase [Candida species]
Immunogen Type: Native Protein
- Isotype
- IgG
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anti-Sucrase Isomaltase, Regulatory (SI-R) antibody
SI-R Reactivity: Candida sp. ELISA, WB Host: Goat Polyclonal unconjugated
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- Application Notes
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Application Note: Anti-Invertase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Invertase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Sucrase Isomaltase, Regulatory (SI-R)
- Alternative Name
- SUC1
- Background
- Background: Anti-Invertase antibody recognizes invertase which hydrolyzes the alpha-1,4-glycosidic bond between alpha-D-glucose and beta-D-fructose of sucrose. Bees use invertase to convert nectar to honey. In the confectionary industry, yeast-derived invertase is used to hydrolyze sucrose to glucose and fructose as fructose is more sweeter and less prone to crystallization. Anti-Invertase antibody is ideal for investigators interested in Metabolism and Cell Biology.
- Gene ID
- 3640338
- UniProt
- P10594
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