AFAP1L1 antibody (N-Term)

Catalog No. ABIN1031218

Product Details anti-AFAP1L1 Antibody

Target: AFAP1L1 (Actin Filament Associated Protein 1-Like 1 (AFAP1L1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFAP1L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.

Purification: Affinity chromatography purified via peptide column

Immunogen: AFAP1L1 antibody was raised against a AA 19 synthetic peptide near the amino terminus of human AFAP1L1.

Isotype: IgG

Application Details

Application Notes: AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: AFAP1L antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.

Expiry Date: 12 months

Target Details for AFAP1L1

Target: AFAP1L1 (Actin Filament Associated Protein 1-Like 1 (AFAP1L1))

Abstract: AFAP1L1 Products

Background: Synonym:
Actin filament associated protein 1-like 1

The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation . Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.

Gene ID: 134265

NCBI Accession: NP_689619