CD99 antibody

Catalog No. ABIN1027667

Product Details anti-CD99 Antibody

Target: CD99

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD99 antibody is un-conjugated

Application: Flow Cytometry (FACS), Cytometry by Time of Flight (CyTOF)

Specificity: The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing´s sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human thymocytes

Clone: 3B2-TA8

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD99 antibody (ABIN1027667)

Brémond, Meynet, Mahiddine, Coito, Tichet, Scotlandi, Breittmayer, Gounon, Gleeson, Bernard, Bernard: "Regulation of HLA class I surface expression requires CD99 and p230/golgin-245 interaction." in: Blood, Vol. 113, Issue 2, pp. 347-57, (2009) (PubMed).

Kueng, Leb, Haiderer, Raposo, Thery, Derdak, Schmetterer, Neunkirchner, Sillaber, Seed, Pickl: "General strategy for decoration of enveloped viruses with functionally active lipid-modified cytokines." in: Journal of virology, Vol. 81, Issue 16, pp. 8666-76, (2007) (PubMed).

Target Details for CD99

Target: CD99

Alternative Name: CD99 (CD99 Products)

Synonyms: 1110061M03Rik antibody, 2410026K10Rik antibody, D4 antibody, Pilr-l antibody, pilr-1 antibody, HBA71 antibody, MIC2 antibody, MIC2X antibody, MIC2Y antibody, MSK5X antibody, CD99 antigen antibody, CD99 molecule (Xg blood group) antibody, CD99 molecule antibody, Cd99 antibody, CD99 antibody

Target Type: Viral Protein

Background: CD99 Molecule (Xg blood group),CD99 is a ubiquitous transmembrane type I sialoglycoprotein of a unique and poorly characterized protein family. CD99 is heavily O-glycosylated and was described as a T cell costimulator and strong activator of integrin-mediated actin cytoskeleton assembly, promoting cell adhesion and homotypic aggregation, immediate arrest on an inflamed vascular endothelium, and cell migration through it. Ligation of CD99 under some conditions can lead to apoptosis. Originally CD99 was described as a human thymus leukemia antigen, an Ewing´s sarcoma-specific membrane marker, and an adhesion molecule involved in spontaneous rosette formation of T cells with erythrocytes.,MIC2, HBA71, blood group Xg

Gene ID: 4267

UniProt: P14209