Rabbit anti-Cat IgM (Heavy Chain) Antibody
-
- Target See all IgM products
- IgM
-
Binding Specificity
- Heavy Chain
-
Reactivity
- Cat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgM µ chain
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgM and Cat Serum. No reaction was observed against Cat IgG.
- Immunogen
-
Immunogen: Cat IgM mu heavy chain
- Isotype
- IgG
-
-
- Application Notes
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Cat IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
-
- Target
- IgM
- Abstract
- IgM Products
- Target Type
- Antibody
- Background
-
Synonyms: rabbit anti-Cat IgM mu Antibody, rabbit anti Cat IgM mu
Background: Anti-Cat IgM antibody specifically detects cat IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Cat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
-
