Gelsolin antibody (N-Term)
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- Target See all Gelsolin (GSN) Antibodies
- Gelsolin (GSN)
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Gelsolin antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- Gelsolin (isoform a)
- Sequence
- PQGRVPEARP NS
- Specificity
- This antibody is expected to recognize isoform a (NP_000168.1) only . Reported variants represent identical protein: NP_001121138.1, NP_001121139.1 Reported variants represent identical protein: NP_001121135.1, NP_937895.1, NP_001121134.1, NP_001121137.1,
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence PQGRVPEARPNS-C, from the N Terminus of the protein sequence according to NP_000168.1, NP_937895.1, NP_001121138.1.
- Isotype
- IgG
- Top Product
- Discover our top product GSN Primary Antibody
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- Application Notes
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Western Blot: Approx 100 kDa band observed in Human Colon, Duodenum, Heart and Skeletal Muscle lysates (calculated MW of 85.7 kDa according to NP_000168.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Gelsolin (GSN)
- Alternative Name
- GSN (GSN Products)
- Background
- GSN, gelsolin, ADF, AGEL, DKFZp313L0718, OTTHUMP00000022011, OTTHUMP00000022014, OTTHUMP00000162202, actin-depolymerizing factor, brevin
- Gene ID
- 2934
- NCBI Accession
- NP_000168, NP_937895, NP_001121138
- Pathways
- Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy
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