RRAD antibody (AA 36-48)

Catalog No. ABIN1019652

Product Details anti-RRAD Antibody

Target: RRAD (Ras-Related Associated with Diabetes (RRAD))

Binding Specificity: AA 36-48

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This RRAD antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: RRAD (aa36-48)

Sequence: HRRSMPVDER DLQ

Specificity: Reported variants represent identical protein: NP_004156.1, NP_001122322.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of the protein sequence according to NP_004156.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 37 kDa band observed in Mouse Heart lysates and no 37 kDa in the KO lysates (calculated MW of 33.2 kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for othe

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-RRAD antibody (ABIN1019652)

Levitan, Manning, Withers, Smith, Shaw, Andres, Sorrell, Satin: "Rad-deletion Phenocopies Tonic Sympathetic Stimulation of the Heart." in: Journal of cardiovascular translational research, Vol. 9, Issue 5-6, pp. 432-444, (2017) (PubMed).

Target Details for RRAD

Target: RRAD (Ras-Related Associated with Diabetes (RRAD))

Alternative Name: RRAD (RRAD Products)

Synonyms: ZGC:63471 antibody, RRAD antibody, RAD antibody, RAD1 antibody, REM3 antibody, Rad antibody, RRAD, Ras related glycolysis inhibitor and calcium channel regulator antibody, Ras-related associated with diabetes antibody, RRAD antibody, Rrad antibody

Background: RRAD, Ras-related associated with diabetes, RAD, RAD1, REM3, GTP-binding protein RAD, OTTHUMP00000174805, RAS (RAD and GEM) like GTP binding 3, ras associated with diabetes

Gene ID: 6236, 56437, 83521

NCBI Accession: NP_004156