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Goat anti-Human IgG (Fc Region) Antibody (Texas Red (TR)) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Catalog No. ABIN101663
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2383
    • 511
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 723
    • 699
    • 602
    • 477
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 82
    • 59
    • 47
    • 28
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1777
    • 1576
    • 572
    • 241
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3434
    • 175
    • 1
    Polyclonal
    Conjugate
    • 588
    • 417
    • 416
    • 381
    • 282
    • 276
    • 243
    • 118
    • 108
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 51
    • 48
    • 44
    • 44
    • 44
    • 41
    • 39
    • 35
    • 32
    • 31
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Texas Red (TR)
    Application
    • 1953
    • 1837
    • 1807
    • 1520
    • 933
    • 909
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 53
    • 52
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Specificity
    IgG (H&L)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Human IgG F(c) fragment

    Isotype
    IgG
    Labeling Ratio
    5.6
  • Application Notes

    Application Note: Anti-Human IgG F(c) Texas Red Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Comment

    Texas Red™ is a registered trademark of Molecular Probes Inc.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Goat Anti Human IgG Fc Antibody Texas red™ Conjugate, Goat Anti-Human IgG F(c) Texas Red™ Conjugation Antibody, Goat Anti Human IgG Fc Fragment Antibody Texas Red™ Conjugation

    Background: Anti-Human IgG F(c) Texas Red Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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