Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

Catalog No. ABIN101567

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: IgG (H&L)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Human IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.0

Application Details

Application Notes:

Application Note: Anti-Human IgG (H&L) Texas Red is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Comment:

Texas Red™ is a registered trademark of Molecular Probes Inc.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

References

Zschüntzsch, Zhang, Klinker, Makosch, Klinge, Malzahn, Brinkmeier, Liebetanz, Schmidt: "Treatment with human immunoglobulin G improves the early disease course in a mouse model of Duchenne muscular dystrophy." in: Journal of neurochemistry, Vol. 136, Issue 2, pp. 351-62, (2016) (PubMed).

Zschüntzsch, Voss, Creus, Sehmisch, Raju, Dalakas, Schmidt: "Provision of an explanation for the inefficacy of immunotherapy in sIBM: Quantitative assessment of inflammation and β-amyloid in the muscle." in: Arthritis and rheumatism, (2012) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit Anti Human IgG Texas Red™ Conjugated Antibody, Rabbit Anti-Human IgG Antibody Texas Red™ Conjugation

Background: Anti-Human IgG (H&L) Texas Red generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.