NIPBL antibody (AA 330-365)

Catalog No. ABIN100654

Product Details anti-NIPBL Antibody

Target: NIPBL (Nipped-B like Protein (NIPBL))

Binding Specificity: AA 330-365

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This NIPBL antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: IDN3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human IDN3 protein.

Characteristics: Synonyms: goat anti-IDN3 Antibody, SCC2, NIPBL, CDLS antibody, Colon tumor susceptibility 2 antibody, Delangin antibody, DKFZp434L1319 antibody, FLJ11203 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-365 of Human IDN3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 304 kDa and 122 kDa.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NIPBL

Target: NIPBL (Nipped-B like Protein (NIPBL))

Alternative Name: IDN3 (NIPBL Products)

Background: Background: IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion, subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesions onto chromatin during S phase. It shares 37 % sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair.  IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine.  Multiple isoforms of the protein have been reported.

Gene ID: 25836, 4760549

UniProt: Q6KC79

Pathways: Sensory Perception of Sound, Stem Cell Maintenance