NIPBL antibody (AA 344-356)

Catalog No. ABIN100654

Product Details anti-NIPBL Antibody

Target: NIPBL (Nipped-B like Protein (NIPBL))

Binding Specificity: AA 344-356

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NIPBL antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 344-356 of Human IDN3.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 304 kDa and 122 kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for NIPBL

Target: NIPBL (Nipped-B like Protein (NIPBL))

Alternative Name: IDN3 (NIPBL Products)

Synonyms: CDLS antibody, CDLS1 antibody, IDN3 antibody, IDN3-B antibody, Scc2 antibody, SCC2W antibody, scc2 antibody, nipbl antibody, scc2-2 antibody, NIPBL antibody, nipbla antibody, wu:fi33d08 antibody, 4921518A06Rik antibody, 4933421G18Rik antibody, C79399 antibody, Idn3 antibody, NIPBL, cohesin loading factor antibody, Nipped-B homolog-like antibody, NIPBL, cohesin loading factor S homeolog antibody, nipped-B homolog b (Drosophila) antibody, nipped-B-like protein A antibody, Nipped-B homolog (Drosophila) antibody, NIPBL antibody, NIPBLL antibody, nipbl.S antibody, LOC427439 antibody, nipblb antibody, LOC100164947 antibody, nipbl antibody, Nipbl antibody

Background: IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion; subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesins onto chromatin during S phase. It shares 37% sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair.  IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine.  Multiple isoforms of the protein have been reported.
Synonyms: CDLS antibody, Colon tumor susceptibility 2 antibody, Delangin antibody, DKFZp434L1319 antibody, FLJ11203 antibody

Gene ID: 25836, 4760549

UniProt: Q6KC79

Pathways: Sensory Perception of Sound, Stem Cell Maintenance