Hub1 antibody
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- Target
- Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
- Reactivity
- Saccharomyces cerevisiae
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), ELISA
- Purpose
- Hub1 Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- Characteristics
- Synonyms: rabbit anti-Hub1 Antibody, rabbit anti-Hub 1 Antibody, Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Immunogen
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Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
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- Application Notes
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Application Note: This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates). Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 5.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
- Alternative Name
- HUB1
- Background
- Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Hub1 (''Homologous to UBiquitin'') may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.
- Gene ID
- 855767, 6324360
- UniProt
- Q6Q546
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