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His Tag antibody

Rockland 600-401-382 WB, ELISA, IHC, DB Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN100492
Supplier Product No.: 600-401-382
  • Target See all His Tag Antibodies
    His Tag
    Reactivity
    Please inquire
    Host
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    Rabbit
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    Polyclonal
    Conjugate
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    This His Tag antibody is un-conjugated
    Application
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
    Supplier Product No.
    600-401-382
    Supplier
    Rockland
    Purpose
    6X His Epitope Tag Antibody
    Sequence
    HHHHHH
    Cross-Reactivity (Details)
    This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.
    Characteristics
    Synonyms: rabbit anti-6X His Epitope Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
    Purification
    Affinity purified antibody
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product His Tag Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

    Western Blot Dilution: 1:500 - 1:1,000

    ELISA Dilution: 1:10,000 - 1:40,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Mart Nez-Cruz, Romero, Hierrezuelo, Thon, de Vicente, P Rez-Garc A: "Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity." in: The Plant cell, Vol. 33, Issue 4, pp. 1319-1340, (2021) (PubMed).

    Wie, Bharthur, Wolfgang, Narayanan, Ramsey, Aranda, Zhang, Zhou, Ren: "Intellectual disability-associated UNC80 mutations reveal inter-subunit interaction and dendritic function of the NALCN channel complex." in: Nature communications, Vol. 11, Issue 1, pp. 3351, (2020) (PubMed).

    Koh, Cao, Mangos, Tardi, Dande, Lee, Samelko, Altintas, Schmitz, Lee, Mukherjee, Peev, Cimbaluk, Reiser, Hahm: "Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist." in: The Journal of clinical investigation, Vol. 129, Issue 4, pp. 1713-1726, (2019) (PubMed).

    Vela-Corcía, Romero, de Vicente, Pérez-García: "Analysis of β-tubulin-carbendazim interaction reveals that binding site for MBC fungicides does not include residues involved in fungicide resistance." in: Scientific reports, Vol. 8, Issue 1, pp. 7161, (2019) (PubMed).

    Lecona, Rodriguez-Acebes, Specks, Lopez-Contreras, Ruppen, Murga, Muñoz, Mendez, Fernandez-Capetillo: "USP7 is a SUMO deubiquitinase essential for DNA replication." in: Nature structural & molecular biology, Vol. 23, Issue 4, pp. 270-7, (2016) (PubMed).

    Lucchetti, Caligiuri, Toffoli, Giordano, Rizzolio: "The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer." in: PLoS ONE, Vol. 8, Issue 2, pp. e55355, (2013) (PubMed).

    La Montagna, Caligiuri, Maranta, Lucchetti, Esposito, Paggi, Toffoli, Rizzolio, Giordano: "Androgen receptor serine 81 mediates Pin1 interaction and activity." in: Cell cycle (Georgetown, Tex.), Vol. 11, Issue 18, pp. 3415-20, (2013) (PubMed).

    Bradley, Chan, Hagood: "Effect of the GPI anchor of human Thy-1 on antibody recognition and function." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 93, Issue 3, pp. 365-74, (2013) (PubMed).

    Curtis, Griffin: "The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling." in: Molecular and cellular biology, Vol. 32, Issue 7, pp. 1312-20, (2012) (PubMed).

    García-López, Sánchez-Amaya, Halm, Astola, Prat et al.: "Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to ..." in: General and comparative endocrinology, Vol. 174, Issue 3, pp. 326-34, (2012) (PubMed).

    Sorrentino, Scheer, Santos, Reits, Bleijlevens, Zelcer: "Distinct functional domains contribute to degradation of the low density lipoprotein receptor (LDLR) by the E3 ubiquitin ligase inducible Degrader of the LDLR (IDOL)." in: The Journal of biological chemistry, Vol. 286, Issue 34, pp. 30190-9, (2011) (PubMed).

    Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." in: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

    Monegal, Ami, Martinelli, Huang, Aliprandi, Capasso, Francavilla, Ossolengo, de Marco: "Immunological applications of single-domain llama recombinant antibodies isolated from a naïve library." in: Protein engineering, design & selection : PEDS, Vol. 22, Issue 4, pp. 273-80, (2009) (PubMed).

    Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, (2005) (PubMed).

  • Target
    His Tag
    Alternative Name
    6X His (His Tag Products)
    Target Type
    Tag
    Background
    Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. 
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