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HIF3A antibody (AA 575-600)

Rockland 600-401-435 HIF3A Reactivity: Mouse WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN100475
Supplier Product No.: 600-401-435
  • Target See all HIF3A Antibodies
    HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))
    Binding Specificity
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 575-600
    Reactivity
    • 23
    • 8
    • 6
    • 1
    Mouse
    Host
    • 20
    • 4
    • 1
    Rabbit
    Clonality
    • 24
    • 1
    Polyclonal
    Conjugate
    • 21
    • 2
    • 1
    • 1
    This HIF3A antibody is un-conjugated
    Application
    • 19
    • 11
    • 10
    • 5
    • 2
    • 2
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-401-435
    Supplier
    Rockland
    Purpose
    Hif3 alpha Antibody
    Cross-Reactivity (Details)
    This affinity-purified antibody is directed against mouse Hif3alpha protein.
    Characteristics
    Synonyms: rabbit anti-Hif3a antibody, Hif3 alpha antibody, Hif3α antibody, Hypoxia Inducible Factor 3-alpha antibody, Hypoxia inducible factor 3 alpha subunit antibody, Inhibitory PAS domain protein antibody, IPAS antibody, HIF-3-alpha, Basic-helix-loop-helix-PAS protein MOP7, HIF3-alpha-1, Neonatal and embryonic PAS protein
    Purification
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 575-600 of mouse Hif3 a (Hypoxia Inducible Factor).

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product HIF3A Primary Antibody
  • Application Notes

    Application Note: This affinity-purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72 kDa in size corresponding to Hif3 alpha protein by western blotting in the appropriate cell lysate or extract. This antibody is expected to cross-react with mouse and rat Hif3 alpha due to sequence homology.

    Western Blot Dilution: 1:1,000 - 1:8,000

    ELISA Dilution: 1:14,000 - 1:80,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.2 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Takeda, Ho, Takeda, Duan, Nagy, Fong: "Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8336-46, (2006) (PubMed).

  • Target
    HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))
    Alternative Name
    Hif3a (HIF3A Products)
    Background
    Background: One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF binds to the hypoxia-responsive elements (HRE) located within the enhancer/promoter of hypoxia-inducible target genes and hence inhibits HRE-driven transcriptional activation. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-a may be a marker for tumor growth and apoptosis. Hif3 may participate in disorders with the cornea, lung, and heart. Anti-Hif3 alpha Antibody is useful for researchers interested in transcription factors, DNA binding, and Epilepsy research.
    Gene ID
    53417, 170295859
    UniProt
    Q0VBL6
    Pathways
    Warburg Effect
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