Hexokinase antibody

Catalog No. ABIN100460

Product Details anti-Hexokinase Antibody

Target: Hexokinase

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hexokinase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Hexokinase from other tissues and species may occur but have not been specifically determined.

Purity: Anti-Hexokinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Hexokinase [Yeast].

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-Hexokinase Antibody was produced by repeated immunizations with yeast hexokinase protein.
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Hexokinase Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:565.000
Western Blot Dilution: 1:500 - 1:2.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

References for anti-Hexokinase antibody (ABIN100460)

Lim, Chang, Huh: "Phosphoregulation of Rad51/Rad52 by CDK1 functions as a molecular switch for cell cycle-specific activation of homologous recombination." in: Science advances, Vol. 6, Issue 6, pp. eaay2669, (2020) (PubMed).

Lam, Snider, Rehal, Wong, Aboualizadeh, Drecun, Wong, Jubran, Li, Ali, Jessulat, Deineko, Miller, Lee, Park, Davidson, Babu, Stagljar: "A Comprehensive Membrane Interactome Mapping of Sho1p Reveals Fps1p as a Novel Key Player in the Regulation of the HOG Pathway in S. cerevisiae." in: Journal of molecular biology, Vol. 427, Issue 11, pp. 2088-103, (2015) (PubMed).

Target Details for Hexokinase

Target: Hexokinase

Abstract: Hexokinase Products

Background: Anti-Hexokinase antibody detects hexokinase. Hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Anti-Hexokinase Antibody is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research. Synonyms: DKFZp686M1669 antibody, Hexokinase 2 antibody, Hexokinase 2 muscle antibody, Hexokinase type II antibody

Gene ID: 852639

UniProt: P04807