AGO6 antibody
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- Target
- AGO6 (Argonaute 6 (AGO6))
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Arabidopsis thaliana
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 116.4 / 99
- Purification
- serum
- Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
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- Application Notes
- 1: 1000 (WB)
- Comment
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antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 μg of sterile water.
- Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- AGO6 (Argonaute 6 (AGO6))
- Alternative Name
- AGO6
- Synonyms
- ARGONAUTE 6 antibody, T21L14.12 antibody, T21L14_12 antibody, Argonaute family protein antibody, AGO6 antibody
- Background
- AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
- Molecular Weight
- expected: 116.4 kDa, apparent: 99
- UniProt
- O48771
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