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AGO6 antibody

AGO6 Reactivity: Arabidopsis thaliana WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN619533
  • Target
    AGO6 (Argonaute 6 (AGO6))
    Reactivity
    Arabidopsis thaliana
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Predicted Reactivity
    Arabidopsis thaliana
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 116.4 / 99
    Purification
    serum
    Immunogen
    KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
  • Application Notes
    1: 1000 (WB)
    Comment

    antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μg of sterile water.
    Storage Comment
    Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    AGO6 (Argonaute 6 (AGO6))
    Alternative Name
    AGO6
    Synonyms
    ARGONAUTE 6 antibody, T21L14.12 antibody, T21L14_12 antibody, Argonaute family protein antibody, AGO6 antibody
    Background
    AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
    Molecular Weight
    expected: 116.4 kDa, apparent: 99
    UniProt
    O48771
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