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- Application
- Flow Cytometry (FACS), Activation (Act)
- Purpose
- Cell Activation Cocktail (without Brefeldin A) is composed of PMA and ionomycin.
- Characteristics
- Cell Activation Cocktail (without Brefeldin A) is a pre-mixed cocktail with optimized concentration of PMA (phorbol 12-myristate-13-acetate) and ionomycin. Activation of cells by PMA and ionomycin leads to an increase in cell surface expression of certain markers such as CD69 and CD154, which can be detected using this cocktail.
- Components
- Cell Activation Cocktail (without Brefeldin A)
- Solubility
- DMSO
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- Comment
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Each vial of this cocktail contains phorbol-12-myristate 13-acetate (40.5 µM) and ionomycin (669.3 µM) in DMSO (500X).
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Each vial of this cocktail contains phorbol-12-myristate 13-acetate (40.5 μM) and ionomycin (669.3 μM) in DMSO (500X).
- Handling Advice
- Protect from light. Avoid repeat freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store cocktail at -70°C upon receipt.
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